Open AccessQuantitative Proteomics Reveals Docosahexaenoic Acid-Mediated Neuroprotective Effects in Lipopolysaccharide-Stimulated Microglial Cells
Author(s) -
Bo Yang,
Yuanli Zhao,
Pei N Liu,
Xin Geng,
Brian Mooney,
Chen Chen,
Kevin L. Fritsche,
David Q. Beversdorf,
James C.-M. Lee,
Grace Y. Sun,
C. Michael Greenlief
Publication year - 2020
Publication title -
journal of proteome research
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.644
H-Index - 161
eISSN - 1535-3907
pISSN - 1535-3893
DOI - 10.1021/acs.jproteome.9b00792
Subject(s) - docosahexaenoic acid , neuroprotection , blot , proteomics , lipopolysaccharide , microbiology and biotechnology , quantitative proteomics , chemistry , microglia , biology , inflammation , biochemistry , polyunsaturated fatty acid , fatty acid , immunology , pharmacology , gene
The high levels of docosahexaenoic acid (DHA) in cell membranes within the brain have led to a number of studies exploring its function. These studies have shown that DHA can reduce inflammatory responses in microglial cells. However, the method of action is poorly understood. Here, we report the effects of DHA on microglial cells stimulated with lipopolysaccharides (LPSs). Data were acquired using the parallel accumulation serial fragmentation method in a hybrid trapped ion mobility-quadrupole time-of-flight mass spectrometer. Over 2800 proteins are identified using label-free quantitative proteomics. Cells exposed to LPSs and/or DHA resulted in changes in cell morphology and expression of 49 proteins with differential abundance (greater than 1.5-fold change). The data provide details about pathways that are influenced in this system including the nuclear factor κ-light-chain-enhancer of the activated B cells (NF-κB) pathway. Western blots and enzyme-linked immunosorbent assay studies are used to help confirm the proteomic results. The MS data are available at ProteomeXchange.