Open AccessGlobal Post-Translational Modification Discovery
Author(s) -
Qiyao Li,
Michael R. Shortreed,
Craig D. Wenger,
Brian L. Frey,
Leah V. Schaffer,
Mark Scalf,
Lloyd M. Smith
Publication year - 2017
Publication title -
journal of proteome research
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.644
H-Index - 161
eISSN - 1535-3907
pISSN - 1535-3893
DOI - 10.1021/acs.jproteome.6b00034
Subject(s) - uniprot , posttranslational modification , proteomics , mass spectrometry , computational biology , database search engine , computer science , chemistry , biology , information retrieval , chromatography , biochemistry , search engine , gene , enzyme
A new global post-translational modification (PTM) discovery strategy, G-PTM-D, is described. A proteomics database containing UniProt-curated PTM information is supplemented with potential new modification types and sites discovered from a first-round search of mass spectrometry data with ultrawide precursor mass tolerance. A second-round search employing the supplemented database conducted with standard narrow mass tolerances yields deep coverage and a rich variety of peptide modifications with high confidence in complex unenriched samples. The G-PTM-D strategy represents a major advance to the previously reported G-PTM strategy and provides a powerful new capability to the proteomics research community.