Open AccessConsiderations for MALDI-Based Quantitative Mass Spectrometry Imaging Studies
Author(s) -
Fernando Tobias,
Amanda B. Hummon
Publication year - 2020
Publication title -
journal of proteome research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.644
H-Index - 161
eISSN - 1535-3907
pISSN - 1535-3893
DOI - 10.1021/acs.jproteome.0c00443
Subject(s) - maldi imaging , mass spectrometry imaging , mass spectrometry , analyte , normalization (sociology) , computer science , chemistry , biological system , chromatography , matrix assisted laser desorption/ionization , biology , organic chemistry , adsorption , desorption , sociology , anthropology
Significant advances in mass spectrometry imaging (MSI) have pushed the boundaries in obtaining spatial information and quantification in biological samples. Quantitative MSI (qMSI) has typically been challenging to achieve because of matrix and tissue heterogeneity, inefficient analyte extraction, and ion suppression effects, but recent studies have demonstrated approaches to obtain highly robust methods and reproducible results. In this perspective, we share our insights into sample preparation, how the choice of matrix influences sensitivity, construction of calibration curves, signal normalization, and visualization of MSI data. We hope that by articulating these guidelines that qMSI can be routinely conducted while retaining the analytical merits of other mass spectrometry modalities.