Open AccessTwo-Photon Excited Polarization-Dependent Autofluorescence of Amyloids as a Label-Free Method of Fibril Organization Imaging
Author(s) -
Patryk Obstarczyk,
Maciej Lipok,
Manuela Grelich-Mucha,
Marek Samoć,
Joanna OlesiakBańska
Publication year - 2021
Publication title -
the journal of physical chemistry letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.563
H-Index - 203
ISSN - 1948-7185
DOI - 10.1021/acs.jpclett.0c03511
Subject(s) - autofluorescence , fibril , thioflavin , amyloid fibril , congo red , chemistry , excited state , two photon excitation microscopy , biophysics , amyloid (mycology) , polarization (electrochemistry) , neurodegeneration , fluorescence , amyloid β , alzheimer's disease , biochemistry , optics , biology , disease , pathology , medicine , physics , atomic physics , inorganic chemistry , adsorption
Amyloids are broadly investigated protein misfolding products with characteristic β-sheet assemblies that have an important role in neurodegenerative diseases (e.g., Alzheimer's disease). While they are usually visualized by staining with Thioflavin-T, Congo Red, or other fluorescent markers, it still arouses a controversy over possible staining molecule influence on the amyloid structure or aggregation process. In this work we present, for the first time, the polarization analysis of two-photon excited autofluorescence of amyloids and confirm that polarization dependence of the observed emission can be correlated with the orientation of fibrils. We show the potential of two-photon excited autofluorescence for resolution of molecular organization of fibrils within amyloid superstructures. This label-free method is compatible with two-photon imaging already applied in investigation of neurodegeneration model in mice.