Open AccessLeaving the Limits of Linearity for Light Microscopy
Author(s) -
Marea J. Blake,
Brandon A. Colon,
Tessa R. Calhoun
Publication year - 2020
Publication title -
journal of physical chemistry. c./journal of physical chemistry. c
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 289
eISSN - 1932-7455
pISSN - 1932-7447
DOI - 10.1021/acs.jpcc.0c07501
Subject(s) - leverage (statistics) , ultrashort pulse , microscopy , nanotechnology , data science , computer science , modalities , atomic force microscopy , perspective (graphical) , artificial intelligence , materials science , optics , physics , sociology , laser , social science
Nonlinear microscopy has enabled additional modalities for chemical contrast, deep penetration into biological tissues, and the ability to collect dynamics on ultrafast timescales across heterogenous samples. The additional light fields introduced to a sample offer seemingly endless possibilities for variation to optimize and customize experimentation and the extraction of physical insight. This perspective highlights three areas of growth in this diverse field: the collection of information across multiple timescales, the selective imaging of interfacial chemistry, and the exploitation of quantum behavior for future imaging directions. Future innovations will leverage the work of the studies reviewed here as well as address the current challenges presented.