Open AccessNew Diagnostic Tool for Ion Channel Activity Hidden Behind the Dwell-Time Correlations
Author(s) -
Przemysław Borys,
Paulina Trybek,
Beata Dworakowska,
P. Bednarczyk,
Agata Wawrzkiewicz-Jałowiecka
Publication year - 2022
Publication title -
the journal of physical chemistry. b
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 392
eISSN - 1520-6106
pISSN - 1520-5207
DOI - 10.1021/acs.jpcb.2c02272
Subject(s) - channel (broadcasting) , dwell time , computer science , gating , ion channel , biological system , molecular dynamics , algorithm , chemistry , biophysics , computational chemistry , biology , telecommunications , clinical psychology , medicine , biochemistry , receptor
The patch-clamp technique is a powerful tool that allows for a long observation of transport protein activity in real time. Experimental traces of single-channel currents can be considered as a record of the channel's conformational switching related to its activation and gating. In this work, we present a mathematically simple method of patch-clamp data analysis that assesses the connectivity and occupancy of distinct conformational substates of the channel. The proposed approach appears to be a big step forward due to its possible applications in the determination of channel substates related to disease and in the analysis of drug-channel interactions on the level of repetitive sequences of channel conformations. This is especially important in cases when molecular dynamics docking is impossible and Markovian modeling requires ambiguous optimization tasks.