Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis | Zendy

Thomas Haas | Zendy; Danye Qiu | Zendy; Markus Häner | Zendy; Larissa Angebauer | Zendy; Alexander Ripp | Zendy; Jyoti Singh | Zendy; HansGeorg Koch | Zendy; Claudia JessenTrefzer | Zendy; Henning J. Jessen | Zendy

Open Access

Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis

Author(s) -

Thomas Haas,

Danye Qiu,

Markus Häner,

Larissa Angebauer,

Alexander Ripp,

Jyoti Singh,

HansGeorg Koch,

Claudia JessenTrefzer,

Henning J. Jessen

Publication year - 2020

Publication title -

the journal of organic chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.2

H-Index - 228

eISSN - 1520-6904

pISSN - 0022-3263

DOI - 10.1021/acs.joc.0c00841

Subject(s) - capillary electrophoresis , nucleotide , reagent , chemistry , phosphoramidite , magic (telescope) , electrophoresis , capillary action , chromatography , biochemistry , dna , materials science , organic chemistry , physics , gene , oligonucleotide , quantum mechanics , composite material

The complex phosphorylation pattern of natural and modified pentaphosphorylated magic spot nucleotides is generated in a highly efficient way. A cyclic pyrophosphoryl phosphoramidite (cPyPA) reagent is used to introduce four phosphates on nucleosides regioselectively in a one-flask key transformation. The obtained magic spot nucleotides are used to develop a capillary electrophoresis UV detection method, enabling nucleotide assignment in complex bacterial extracts.

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