Open AccessStructure and Candidate Biosynthetic Gene Cluster of a Manumycin-Type Metabolite from Salinispora pacifica
Author(s) -
Gabriel CastroFalcón,
Kaitlin E. Creamer,
Alexander B. Chase,
Min Cheol Kim,
Douglas Sweeney,
Evgenia Glukhov,
William Fenical,
Paul R. Jensen
Publication year - 2022
Publication title -
journal of natural products
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.976
H-Index - 139
eISSN - 1520-6025
pISSN - 0163-3864
DOI - 10.1021/acs.jnatprod.1c01117
Subject(s) - gene cluster , gene , secondary metabolite , natural product , biology , cluster (spacecraft) , phylum , metabolite , genetics , botany , biochemistry , computer science , programming language
A new manumycin-type natural product named pacificamide ( 1 ) and its candidate biosynthetic gene cluster ( pac ) were discovered from the marine actinobacterium Salinispora pacifica CNT-855. The structure of the compound was determined using NMR, electronic circular dichroism, and bioinformatic predictions. The pac gene cluster is unique to S. pacifica and found in only two of the 119 Salinispora genomes analyzed across nine species. Comparative analyses of biosynthetic gene clusters encoding the production of related manumycin-type compounds revealed genetic differences in accordance with the unique pacificamide structure. Further queries of manumycin-type gene clusters from public databases revealed their limited distribution across the phylum Actinobacteria and orphan diversity that suggests additional products remain to be discovered in this compound class. Production of the known metabolite triacsin D is also reported for the first time from the genus Salinispora . This study adds two classes of compounds to the natural product collective isolated from the genus Salinispora , which has proven to be a useful model for natural product research.