Open AccessDiterpenoid Constituents of Psiadia punctulata and Evaluation of Their Antimicrobial Activity
Author(s) -
Giuliana Donadio,
Maria Giovanna Chini,
Valentina Parisi,
Francesca Mensitieri,
Nicola Malafronte,
Giuseppe Bifulco,
Angela Bisio,
Nunziatina De Tommasi,
Ammar Bader
Publication year - 2022
Publication title -
journal of natural products
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.976
H-Index - 139
eISSN - 1520-6025
pISSN - 0163-3864
DOI - 10.1021/acs.jnatprod.1c01093
Subject(s) - diterpene , labdane , terpenoid , antimicrobial , chemistry , antiseptic , staphylococcus aureus , stereochemistry , traditional medicine , antibacterial activity , biology , organic chemistry , bacteria , medicine , genetics
Sixteen diterpenes ( 1 - 16 ), along with 10 previously described compounds, including four flavonoids and six diterpenes, were isolated from the aerial parts of Psiadia punctulata growing in Saudi Arabia. The diterpene structures were elucidated using NMR spectroscopy and mass spectrometry data. Furthermore, a DFT/NMR procedure was used to suggest the relative configuration of several compounds. The labdane-derived skeletons, namely, ent -atisane, ent -beyerene, ent -trachylobane, and ent -kaurene, were identified. The extracts, fractions, and pure compounds were then tested against Staphylococcus aureus , Streptococcus mutans , Treponema denticola , and Lactobacillus plantarum . One diterpenoid, namely, psiadin, showed an additive effect with the antiseptic chlorhexidine, with a fractional inhibitory concentration index of less than 1. Additionally, psiadin showed a prospective inhibition activity for bacterial efflux pumps.