Open AccessPrenylated Coumaric Acids from Artemisia scoparia Beneficially Modulate Adipogenesis
Author(s) -
David M. Ribnicky,
Seon Beom Kim,
Alexander Poulev,
Yan Wang,
Anik Boudreau,
Ilya Raskin,
Jonathan Bisson,
G. Joseph Ray,
Shao-g Chen,
Allison J. Richard,
Jacqueline M. Stephens,
Guido F. Pauli
Publication year - 2021
Publication title -
journal of natural products
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.976
H-Index - 139
eISSN - 1520-6025
pISSN - 0163-3864
DOI - 10.1021/acs.jnatprod.0c01149
Subject(s) - chemistry , fractionation , scoparia , artemisia , artemisia annua , p coumaric acid , oil red o , adipocyte , biochemistry , adipogenesis , chromatography , biology , botany , ferulic acid , gene , adipose tissue , artemisinin , malaria , immunology , plasmodium falciparum
Two new diprenylated coumaric acid isomers ( 1a and 1b ) and two known congeners, capillartemisin A ( 2 ) and B ( 3 ), were isolated from Artemisia scoparia as bioactive markers using bioactivity-guided HPLC fractionation. Their structures were determined by spectroscopic means, including 1D and 2D NMR methods and LC-MS, with their purity assessed by 1D 1 H pure shift qNMR spectroscopic analysis. The bioactivity of compounds was evaluated by enhanced accumulation of lipids, as measured using Oil Red O staining, and by increased expression of several adipocyte marker genes, including adiponectin in 3T3-L1 adipocytes relative to untreated negative controls. Compared to the plant's 80% EtOH extract, these purified compounds showed significant but still weaker inhibition of TNFα-induced lipolysis in 3T3-L1 adipocytes. This suggests that additional bioactive substances are responsible for the multiple metabolically favorable effects on adipocytes observed with Artemisia scoparia extract.