Open AccessMycoplasma Infection Mediates Sensitivity of Multidrug-Resistant Cell Lines to Tiopronin: A Cautionary Tale
Author(s) -
Lyn M. Huff,
Sachi Horibata,
Robert W. Robey,
Matthew D. Hall,
Michael M. Gottesman
Publication year - 2019
Publication title -
journal of medicinal chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.01
H-Index - 261
eISSN - 1520-4804
pISSN - 0022-2623
DOI - 10.1021/acs.jmedchem.9b00484
Subject(s) - tiopronin , multiple drug resistance , mycoplasma , chemistry , in vitro , microbiology and biotechnology , cell culture , efflux , drug resistance , virology , biology , pharmacology , biochemistry , genetics
We previously reported that some, but not all, multidrug-resistant cells that overexpressed various drug-resistance transporters were collaterally sensitive to tiopronin. In recent follow-up studies, we discovered that sensitivity to tiopronin in the original study was mediated by infection of the cells by a human-specific strain of mycoplasma. These results strongly support the need to constantly monitor cells for mycoplasma infection and keep stored samples of all cells that are used for in vitro studies.