Measurement of Ligand–Target Residence Times by 1H Relaxation Dispersion NMR Spectroscopy | Zendy

Thomas Moschen | Zendy; Sarina Grutsch | Zendy; Michael Andreas Juen | Zendy; Christoph H. Wunderlich | Zendy; Christoph Kreutz | Zendy; Martin Tollinger | Zendy

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Measurement of Ligand–Target Residence Times by ¹H Relaxation Dispersion NMR Spectroscopy

Author(s) -

Thomas Moschen,

Sarina Grutsch,

Michael Andreas Juen,

Christoph H. Wunderlich,

Christoph Kreutz,

Martin Tollinger

Publication year - 2016

Publication title -

journal of medicinal chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.01

H-Index - 261

eISSN - 1520-4804

pISSN - 0022-2623

DOI - 10.1021/acs.jmedchem.6b01110

Subject(s) - chemistry , relaxation (psychology) , ligand (biochemistry) , nuclear magnetic resonance spectroscopy , millisecond , dispersion (optics) , nucleic acid , isotope , kinetics , molecule , residence time (fluid dynamics) , spectroscopy , analytical chemistry (journal) , computational chemistry , chemical physics , stereochemistry , chromatography , organic chemistry , biochemistry , receptor , psychology , social psychology , physics , geotechnical engineering , quantum mechanics , astronomy , optics , engineering

A ligand-observed 1 H NMR relaxation experiment is introduced for measuring the binding kinetics of low-molecular-weight compounds to their biomolecular targets. We show that this approach, which does not require any isotope labeling, is applicable to ligand-target systems involving proteins and nucleic acids of variable molecular size. The experiment is particularly useful for the systematic investigation of low affinity molecules with residence times in the micro- to millisecond time regime.

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