Respiratory Vinyl Chloride Reductive Dechlorination to Ethene in TceA-Expressing Dehalococcoides mccartyi | Zendy

Jun Yan | Zendy; Jingjing Wang | Zendy; Manuel I. Villalobos Solis | Zendy; Huijuan Jin | Zendy; Karuna Chourey | Zendy; Xiuying Li | Zendy; Yi Yang | Zendy; Yongchao Yin | Zendy; Robert L. Hettich | Zendy; Frank E. Löffler | Zendy

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Respiratory Vinyl Chloride Reductive Dechlorination to Ethene in TceA-Expressing Dehalococcoides mccartyi

Author(s) -

Jun Yan,

Jingjing Wang,

Manuel I. Villalobos Solis,

Huijuan Jin,

Karuna Chourey,

Xiuying Li,

Yi Yang,

Yongchao Yin,

Robert L. Hettich,

Frank E. Löffler

Publication year - 2021

Publication title -

environmental science and technology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.851

H-Index - 397

eISSN - 1520-5851

pISSN - 0013-936X

DOI - 10.1021/acs.est.0c07354

Subject(s) - vinyl chloride , reductive dechlorination , strain (injury) , dehalogenase , chemistry , dehalococcoides , chlorine , electron acceptor , electron donor , bioremediation , enrichment culture , microbiology and biotechnology , biodegradation , nuclear chemistry , gene , biology , biochemistry , bacteria , organic chemistry , catalysis , genetics , copolymer , anatomy , polymer

Bioremediation of chlorinated ethenes in anoxic aquifers hinges on organohalide-respiring Dehalococcoidia expressing vinyl chloride (VC) reductive dehalogenase (RDase). The ceA gene encoding the trichloroethene-dechlorinating RDase TceA is frequently detected in contaminated groundwater but not recognized as a biomarker for VC detoxification. We demonstrate tha ceA -carrying Dehalococcoides mccartyi ( Dhc ) strains FL2 and 195 grow with VC as an electron acceptor when sufficient vitamin B 12 (B 12 ) is provided. Strain FL2 cultures that received 50 μg L -1 B 12 completely dechlorinated VC to ethene at rates of 14.80 ± 1.30 μM day -1 and attained 1.64 ± 0.11 × 10 8 cells per μmol of VC consumed. Strain 195 attained similar growth yields of 1.80 ± 1.00 × 10 8 cells per μmol of VC consumed, and both strains could be consecutively transferred with VC as the electron acceptor. Proteomic analysis demonstrated TceA expression in VC-grown strain FL2 cultures. Resequencing of the strain FL2 and strain 195 ceA genes identified non-synonymous substitutions, although their consequences for TceA function are currently unknown. The finding tha Dhc strains expressing TceA respire VC can explain ethene formation at chlorinated solvent sites, where quantitative polymerase chain reaction analysis indicates tha ceA dominates the RDase gene pool.

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