z-logo
open-access-imgOpen Access
Polymer Coatings on Virus-like Particle Nanoreactors at Low Ionic Strength—Charge Reversal and Substrate Access
Author(s) -
Pawel Kraj,
Ekaterina Selivanovitch,
Byeongdu Lee,
Trevor Douglas
Publication year - 2021
Publication title -
biomacromolecules
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.689
H-Index - 220
eISSN - 1526-4602
pISSN - 1525-7797
DOI - 10.1021/acs.biomac.1c00208
Subject(s) - nanoreactor , ionic strength , polymer , materials science , macromolecule , ionic bonding , coating , chemical engineering , substrate (aquarium) , polyelectrolyte , nanotechnology , smart polymer , polymer chemistry , nanoparticle , chemistry , composite material , ion , organic chemistry , aqueous solution , biochemistry , oceanography , geology , engineering
Virus-like particles (VLPs) are a class of biomaterials which serve as platforms for achieving the desired functionality through interior and exterior modifications. Through ionic strength-mediated electrostatic interactions, VLPs have been assembled into hierarchically ordered materials. This work builds on predictive models to prepare polymer-coated VLP clusters at very low ionic strength. Zeta potential measurements showed that the clusters carried a strongly positive charge, a complete charge reversal from the VLP building block. SAXS analysis confirmed polymer adsorption onto the VLP exterior. We then studied the activity of an encapsulated enzyme toward small molecular and macromolecular substrates to determine the effect of each component of the hierarchically assembled material. We found that while encapsulation and polymer coating did not have a large effect on access to the enzyme by its native, small molecular substrate, substrate modification with a macromolecule caused the polymer coating and encapsulation to affect the access to the enzyme.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here