Open AccessDirect N- or C-Terminal Protein Labeling Via a Sortase-Mediated Swapping Approach
Author(s) -
Cong Ma,
Soheil Tavakolpour,
Léa Berland,
Hannah J. Glockner,
Bohdan Andreiuk,
Taha Rakhshandehroo,
Safak Uslu,
Shruti Mishra,
Louise Clark,
Mohammad Rashidian
Publication year - 2021
Publication title -
bioconjugate chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.279
H-Index - 172
eISSN - 1520-4812
pISSN - 1043-1802
DOI - 10.1021/acs.bioconjchem.1c00442
Subject(s) - sortase , chemistry , sortase a , cleavage (geology) , posttranslational modification , computational biology , terminal (telecommunication) , fusion protein , biochemistry , combinatorial chemistry , bacterial protein , recombinant dna , gene , computer science , enzyme , telecommunications , geotechnical engineering , fracture (geology) , biology , engineering
Site-specific protein labeling is important in biomedical research and biotechnology. While many methods allow site-specific protein modification, a straightforward approach for efficient N-terminal protein labeling is not available. We introduce a novel sortase-mediated swapping approach for a one-step site-specific N-terminal labeling with a near-quantitative yield. We show that this method allows rapid and efficient cleavage and simultaneous labeling of the N or C termini of fusion proteins. The method does not require any prior modification beyond the genetic incorporation of the sortase recognition motif. This new approach provides flexibility for protein engineering and site-specific protein modifications.