Flow-Free Microfluidic Device for Quantifying Chemotaxis in Spermatozoa | Zendy

Johanna Theodora Wilhelmina Berendsen | Zendy; Stella A. Kruit | Zendy; Nihan Atak | Zendy; Ellen Willink | Zendy; Loes I. Segerink | Zendy

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Flow-Free Microfluidic Device for Quantifying Chemotaxis in Spermatozoa

Author(s) -

Johanna Theodora Wilhelmina Berendsen,

Stella A. Kruit,

Nihan Atak,

Ellen Willink,

Loes I. Segerink

Publication year - 2020

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/acs.analchem.9b05183

Subject(s) - spermatozoon , chemistry , chemotaxis , microfluidics , agarose , motility , semen , chemotaxis assay , human fertilization , flow (mathematics) , boar , andrology , biophysics , microbiology and biotechnology , chromatography , nanotechnology , biochemistry , anatomy , mechanics , biology , medicine , materials science , receptor , physics

Current male fertility diagnosis tests focus on assessing the quality of semen samples by studying the concentration, total volume, and motility of spermatozoa. However, other characteristics such as the chemotactic ability of a spermatozoon might influence the chance of fertilization. Here we describe a simple, easy to fabricate and handle, flow-free microfluidic chip to test the chemotactic response of spermatozoa made out of a hybrid hydrogel (8% gelatin/1% agarose). A chemotaxis experiment with 1 μM progesterone was performed that significantly demonstrated that boar spermatozoa are attracted by a progesterone gradient.

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