Open AccessPoint-of-Care Tissue Analysis Using Miniature Mass Spectrometer
Author(s) -
Rong Zou,
Wenbo Cao,
Leelyn Chong,
Wei Hua,
Hao Xu,
Ying Mao,
Hang-Eun Joe,
Riyi Shi,
Yu Xia,
Tony Hu,
Wenpeng Zhang,
Zheng Ouyang
Publication year - 2018
Publication title -
analytical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.117
H-Index - 332
eISSN - 1520-6882
pISSN - 0003-2700
DOI - 10.1021/acs.analchem.8b04935
Subject(s) - chemistry , mass spectrometry , ionization , spectrometer , ambient ionization , analytical chemistry (journal) , chromatography , brain tissue , sampling (signal processing) , ion , chemical ionization , biomedical engineering , optics , medicine , physics , organic chemistry , detector
The combination of direct sampling ionization and miniature mass spectrometer presents a promising technical pathway of point-of-care analysis in clinical applications. In this work, a miniature mass spectrometry system was used for analysis of tissue samples. Direct tissue sampling coupled with extraction spray ionization was used with a home-built miniature mass spectrometer, Mini 12. Lipid species in tissue samples were well profiled in rat brain, kidney, and liver in a couple of minutes. By incorporating a photochemical (Paternò-Büchi) reaction, fast identification of lipid C═C location was realized. Relative quantitation of the lipid C═C isomer was performed by calculating the intensity ratio C═C diagnostic product ions, by which FA 18:1 (Δ9)/FA 18:1 (Δ11) was found to change significantly in mouse cancerous breast tissue samples. Accumulation of 2-hydroxylglutarate in human glioma samples, not in normal brains, can also be easily identified for rapid diagnosis.