Single-Cell Chemical Proteomics (SCCP) Interrogates the Timing and Heterogeneity of Cancer Cell Commitment to Death | Zendy

Ákos Végvári | Zendy; Jimmy Rodriguez Murillo | Zendy; Roman A. Zubarev | Zendy

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Single-Cell Chemical Proteomics (SCCP) Interrogates the Timing and Heterogeneity of Cancer Cell Commitment to Death

Author(s) -

Ákos Végvári,

Jimmy Rodriguez Murillo,

Roman A. Zubarev

Publication year - 2022

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/acs.analchem.2c00413

Subject(s) - proteomics , proteome , chemistry , camptothecin , programmed cell death , computational biology , cell , cancer cell , single cell analysis , cancer , cancer research , microbiology and biotechnology , apoptosis , biochemistry , biology , genetics , gene

Chemical proteomics studies the effects of drugs upon a cellular proteome. Due to the complexity and diversity of tumors, the response of cancer cells to drugs is also heterogeneous, and thus, proteome analysis at the single-cell level is needed. Here, we demonstrate that single-cell proteomics techniques have become quantitative enough to tackle the drug effects on target proteins, enabling single-cell chemical proteomics (SCCP). Using SCCP, we studied here the time-resolved response of individual adenocarcinoma A549 cells to anticancer drugs methotrexate, camptothecin, and tomudex, revealing the early emergence of cellular subpopulations committed and uncommitted to death. As a novel and useful approach to exploring the heterogeneous response to drugs of cancer cells, SCCP may prove to be a breakthrough application for single-cell proteomics.

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