Open AccessAssessing Collision Cross Section Calibration Strategies for Traveling Wave-Based Ion Mobility Separations in Structures for Lossless Ion Manipulations
Author(s) -
Ailin Li,
Christopher Conant,
Xueyun Zheng,
Kent Bloodsworth,
Daniel J. Orton,
Sandilya Garimella,
Isaac K. Attah,
Gabe Nagy,
Richard Smith,
Yehia Ibrahim
Publication year - 2020
Publication title -
analytical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.117
H-Index - 332
eISSN - 1520-6882
pISSN - 0003-2700
DOI - 10.1021/acs.analchem.0c02829
Subject(s) - chemistry , ion mobility spectrometry , calibration , waveform , ion , calibration curve , analytical chemistry (journal) , mass spectrometry , sine wave , collision , lossless compression , chromatography , algorithm , physics , voltage , computer security , organic chemistry , quantum mechanics , data compression , computer science , detection limit
The collision cross section (CCS) is an important property that aids in the structural characterization of molecules. Here, we investigated the CCS calibration accuracy with traveling wave ion mobility spectrometry (TWIMS) separations in structures for lossless ion manipulations (SLIM) using three sets of calibrants. A series of singly negatively charged phospholipids and bile acids were calibrated in nitrogen buffer gas using two different TW waveform profiles (square and sine) and amplitudes (20, 25, and 30 V 0-p ). The calibration errors for the three calibrant sets (Agilent tuning mixture, polyalanine, and one assembled in-house) showed negligible differences using a sine-shaped TW waveform. Calibration errors were all within 1-2% of the drift tube ion mobility spectrometry (DTIMS) measurements, with lower errors for sine waveforms, presumably due to the lower average and maximum fields experienced by ions. Finally, ultrahigh-resolution multipass (long path length) SLIM TWIMS separations demonstrated improved CCS calibration for phospholipid and bile acid isomers.