Open AccessBarcoded Microchips for Biomolecular Assays
Author(s) -
Yi Zhang,
Jiashu Sun,
Yu Zou,
Wenwen Chen,
Wei Zhang,
Jianzhong Xi,
Xingyu Jiang
Publication year - 2014
Publication title -
analytical chemistry
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 2.117
H-Index - 332
eISSN - 1520-6882
pISSN - 0003-2700
DOI - 10.1021/ac5032379
Subject(s) - barcode , encode , chemistry , analyte , computational biology , nucleic acid , oligonucleotide , microfluidics , microfluidic chip , human immunodeficiency virus (hiv) , immunoassay , chromatography , nanotechnology , antibody , dna , computer science , virology , biochemistry , biology , genetics , gene , materials science , operating system
Multiplexed assay of analytes is of great importance for clinical diagnostics and other analytical applications. Barcode-based bioassays with the ability to encode and decode may realize this goal in a straightforward and consistent manner. We present here a microfluidic barcoded chip containing several sets of microchannels with different widths, imitating the commonly used barcode. A single barcoded microchip can carry out tens of individual protein/nucleic acid assays (encode) and immediately yield all assay results by a portable barcode reader or a smartphone (decode). The applicability of a barcoded microchip is demonstrated by human immunodeficiency virus (HIV) immunoassays for simultaneous detection of three targets (anti-gp41 antibody, anti-gp120 antibody, and anti-gp36 antibody) from six human serum samples. We can also determine seven pathogen-specific oligonucleotides by a single chip containing both positive and negative controls.
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