Open AccessDetection and Quantification of Early-Stage Malaria Parasites in Laboratory Infected Erythrocytes by Attenuated Total Reflectance Infrared Spectroscopy and Multivariate Analysis
Author(s) -
Aazam Khoshmanesh,
Matthew W. A. Dixon,
Shan Kenny,
Leann Tilley,
Donald McNaughton,
Bayden Robert Wood
Publication year - 2014
Publication title -
analytical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.117
H-Index - 332
eISSN - 1520-6882
pISSN - 0003-2700
DOI - 10.1021/ac500199x
Subject(s) - attenuated total reflection , parasitemia , chemistry , malaria , gametocyte , spectroscopy , detection limit , chromatography , plasmodium falciparum , infrared spectroscopy , immunology , biology , physics , organic chemistry , quantum mechanics
New diagnostic modalities for malaria must have high sensitivity and be affordable to the developing world. We report on a method to rapidly detect and quantify different stages of malaria parasites, including ring and gametocyte forms, using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FT-IR) and partial least-squares regression (PLS). The absolute detection limit was found to be 0.00001% parasitemia (<1 parasite/μL of blood; p < 0.008) for cultured early ring stage parasites in a suspension of normal erythrocytes. Future development of universal and robust calibration models can significantly improve malaria diagnoses, leading to earlier detection and treatment of this devastating disease.