Open AccessStructural Analysis of Glycans by NMR Chemical Shift Prediction
Author(s) -
Magnus Lundborg,
Göran Widmalm
Publication year - 2011
Publication title -
analytical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.117
H-Index - 332
eISSN - 1520-6882
pISSN - 0003-2700
DOI - 10.1021/ac1032534
Subject(s) - chemistry , glycan , glycoconjugate , chemical shift , carbon 13 nmr , oligosaccharide , nuclear magnetic resonance spectroscopy , polysaccharide , chemical structure , stereochemistry , organic chemistry , biochemistry , glycoprotein
Structural determination of N- and O-linked glycans as well as polysaccharides is hampered by the limited spectral dispersion. The computerized approach CASPER, an acronym for computer assisted spectrum evaluation of regular polysaccharides, uses liquid state NMR data to elucidate carbohydrate structure based on agreement with predicted (1)H and (13)C chemical shifts. We here demonstrate developments based on multiple through-bond J-based correlations that significantly enhance the credence to the sequence connectivities proposed in the analysis exemplified by an oligosaccharide and a bacterial polysaccharide. The approach is also suitable for predicting (1)H and (13)C NMR chemical shifts of synthesized oligosaccharides and glycoconjugates, thereby corroborating a proposed structure.