Open AccessApogamous sporophyte development through spore reproduction of a South Asia's critically endangered fern: Pteris tripartita Sw.
Author(s) -
Baskaran Xavier Ravi,
Geo Vigila Antony Varuvel,
Rajan kilimas,
Jeyachandran Robert
Publication year - 2015
Publication title -
asian pacific journal of reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.167
H-Index - 17
eISSN - 2305-0519
pISSN - 2305-0500
DOI - 10.1016/s2305-0500(15)30010-5
Subject(s) - sporophyte , fern , biology , botany , critically endangered , spore , population , shoot , horticulture , endangered species , ecology , habitat , demography , sociology
Objective: To develop an efficient reproducible protocol for a critically endangered fern, Pteris tripartita (P. tripartita) Sw. through spore culture.Methods: The spore derived gametophytes were grown in a half strength MS medium which sub-cultured after three months to MS medium containing growth regulators like BAP, Kinetin and GA3 with 3% sucrose.Results: The highest mean number of sporophytes (64.40) induced with 4.16 cm of length and also formed 1.51 cm of root length at 4 mg/L of BAP. On MS medium supplemented with KIN, 56.03 mean numbers of sporophytes with 2.61 cm along with 1.39 cm length of root were obtained in 4 mg/L of KIN. And, the highest mean numbers of juvenile sporophytes (59.66) were induced with 2.64 cm mean lengths of sporophytes with 2.23 cm of root length in 4 mg/L of GA3 in the MS culture medium. The in vitro developed plantlets were washed with distilled water and grown in small cups containing sterilized soil, sand and vermiculite (1:1:1). The acclimatized plantlet showed 90% of a survival rate.Conclusions: In this study, reproducible protocol could be useful for their rehabilitation and also to reduce the pressure on natural population in Asian habitat