Open AccessAssociation of polymorphism in cell death pathway gene FASLG with human male infertility
Author(s) -
Deepika Jaiswal,
Sameer Trivedi,
N K Agrawal,
Kiran Singh
Publication year - 2015
Publication title -
asian pacific journal of reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.167
H-Index - 17
eISSN - 2305-0519
pISSN - 2305-0500
DOI - 10.1016/s2305-0500(15)30006-3
Subject(s) - genotyping , single nucleotide polymorphism , genotype , snp , infertility , restriction fragment length polymorphism , genetics , male infertility , biology , allele , gene , pregnancy
Objective: To investigate –844C>T single nucleotide polymorphism (SNP) present in the promoter of cell death pathway gene FASLG with male infertile phenotype.Methods: Genotyping for SNP FASLG (rs763110) was done by polymerase chain reaction followed by analysis with specific endonuclease (PCR-RFLP). DNA sequencing was used to ascertain PCR-RFLP results.Results: FASLG –844C>T polymorphism, allele and genotype distribution did not differ significantly between patients and controls (OR: 1.03, 95% CI= 0.7638 to 1.3952, P=0.83). Thus SNP-844C>T of the FASLG gene is not associated with male infertility risk in the analyzed patients.Conclusions: Human male infertility is a complex disorder and thus other genetic or environmental factors may be contributing to the complex etiology, and further study in other region of Indian populations will verify whether it is associated with male infertility risk