Open AccessEffect of different polarities leaves crude extracts of Omani juniperus excels on antioxidant, antimicrobial and cytotoxic activities and their biochemical screening
Author(s) -
Afaf Mohammed Weli,
Jamila R.K. AL-Hinai,
Jawaher M.A. Al-Mjrafi,
Jawaher R.S. Alnaaimi,
M. Amzad Hossain,
Sadri Saeed,
Md. S. Aktar
Publication year - 2014
Publication title -
asian pacific journal of reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.167
H-Index - 17
eISSN - 2305-0519
pISSN - 2305-0500
DOI - 10.1016/s2305-0500(14)60029-4
Subject(s) - phytochemical , antimicrobial , dpph , brine shrimp , traditional medicine , antioxidant , ethyl acetate , chemistry , chloroform , agar diffusion test , antibacterial activity , food science , biology , bacteria , chromatography , biochemistry , medicine , organic chemistry , genetics
Objective: To prepare different polarities leave crude extracts of Juniperus excels (J. excels) and to determine their phytochemical screening, antioxidant, antimicrobial and cytotoxic activities.Methods: The phytochemical screening of different crude extracts was determined by well-established methods. The antioxidant activity was determined by free radical scavenging (2,2-diphenyl-1-picrylhydrazyl, DPPH) method. The antimicrobial activity was evaluated by agar disc diffusion method and cytotoxic activity was determined through brine shrimp lethality assay.Results: The phytochemical tests showed that alkaloids, flavonoids, saponins, steroide, triterpenoids and tannins are present in all leave crude extracts except chloroform crude extract do not contain tannins. The antioxidant results of crude extracts were found to be in the order of hydro alcoholic > chloroform> ethyl acetate >hexane. All leave crude extracts showed moderate antibacterial against gram positive and gram negative food borne pathogen bacteria. The cytotoxicity results revealed that hexane and chloroform extracts have killed all the shrimp larvae at the concentration of 500 μg/mL.Conclusion: More in-vivo and in-vitro studies along with detailed phytochemical investigations are needed in order to potentially use this plant in the prevention and therapies of some oxidative damage related diseases