Open AccessStudies on effect of different seasons on expression of HSP70 and HSP90 gene in sperm of Tharparkar bull semen
Author(s) -
J. S. Rajoriya,
J.K. Prasad,
Subrata Ghosh,
P. Perumal,
Anuj Kumar,
S. Kaushal,
Suman Ramteke
Publication year - 2014
Publication title -
asian pacific journal of reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.167
H-Index - 17
eISSN - 2305-0519
pISSN - 2305-0500
DOI - 10.1016/s2305-0500(14)60025-7
Subject(s) - semen , breed , biology , andrology , sperm , hsp70 , summer season , cryopreservation , extender , winter season , zoology , gene , heat shock protein , botany , anatomy , chemistry , genetics , medicine , embryo , physics , organic chemistry , climatology , meteorology , polyurethane , geology
Objective: To assess the effect of different seasons on expression of HSP70 and HSP90 genes of spermatozoa in Indian breed, Tharparkar bull.Methods: Total numbers of 60 ejaculates from 3 bulls were collected through artificial vagina method twice a week during summer and winter season (30 ejaculates from each season). The semen samples were pooled and diluted with the standard TEYC extender and these semen samples were allowed to study the expression of HSP 70 and HSP 90 genes of spermatozoa with commercially available kit.Results: No significant difference was observed in spermatozoal mRNA expression of HSP 70 and HSP 90 during winter and summer season in this bull semen. But the mRNA expression of both HSP 70 and HSP 90 during summer season was found non-significantly higher in comparison to winter season.Conclusion: It was concluded from the present study that there was no significant difference in the mRNA expression of HSP 70 and HSP 90 between the winter and summer season, presence of similar type of stress resistant spermatozoa in Tharparkar bull semen and the semen can be cryopreserved throughout the year in this prestigious Indian breed