Open AccessChemical characterization and insecticidal activity of Calotropis gigantea L. flower extract against Tribolium castaneum (Herbst)
Author(s) -
M. Rowshanul Habib,
Muhammad Rezaul Karim
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(16)61171-4
Subject(s) - bioassay , pest analysis , larva , biology , gigantea , toxicology , instar , insect , botany , horticulture , genetics
Objective: To test the insecticidal activity of ethyl acetate extract of Calotropis gigantea\udL. flower (designated as EECF) against stored grain pest Tribolium castaneum (Herbst) of\uddifferent larval and adult stages.\udMethods: Residual film method was used here to study the toxicity of EECF against Tribolium\udcastaneum and gas chromatography-mass spectrometer analysis was also performed to\udcharacterize the chemicals of EECF.\udResults: In residual film bioassay, EECF showed lowest LD50 (0.134 mg/cm2) against 1st instar\udlarvae of Tribolium castaneum and this finding ultimately revealed that the insect of initial\udstage was more susceptible than other stages. From the results of this study, it was found that\udwith the increasing of age, Tribolium castaneum showed some extent of resistance against the\udtoxicity of EECF. Moreover, chemical profiles of EECF identified by gas chromatography-mass\udspectrometer analysis were also found to consistent with its insecticidal activity.\udConclusions: So, the overall results suggested that extracts of Calotropis gigantea L. flower\udhave potential insecticidal effect which might be used in pest control