Open AccessIn vitro screening of BTP-Iso on Schistosoma mansoni and its intermediate host Biomphalaria alexandrina
Author(s) -
Amira Taman,
Samar N. El-Beshbishi,
Samia El Bardicy,
Menerva Tadros,
Magda Ayoub,
Basem Mansour,
Serry A. A. El-Bialy
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(16)61162-3
Subject(s) - schistosoma mansoni , snail , schistosomiasis , biology , viral tegument , intermediate host , incubation , biomphalaria , in vitro , toxicology , schistosoma , host (biology) , immunology , helminths , ecology , biochemistry , virology
Objective: To test the effect of benzimidazole-derived compound (compound BTP-Iso) on\udcultured adult Schistosoma mansoni and clean Biomphalaria alexandrina snails.\udMethods: Adult schistosomes were incubated at different concentrations of compound BTP-Iso\udto calculate mortality rate, LC90 and LC50, in addition, tegumental changes were recorded using\udSEM. Clean Biomphalaria alexandrina snails were also incubated with compound BTP-Iso at\udvarious concentrations to report snail mortality.\udResults: Compound BTP-Iso was found to possess potent antischistosomal activities on adult\udworms but no effect was recorded on snail host. At a concentration of 8 µg/mL, mortality rates\udwere 45.8% and 81.0% after 48 h and 72 h incubation, respectively, while 100% mortality was\udrecorded after 48 h incubation at 20.0 µg/mL and after 72 h incubation at 10.0 µg/mL, with LC50\udand LC\ud90 of 6.1 µg/mL and 9.8 µg/mL, respectively. Morphological changes and tegumental\udalternation of treated worms suggested loss of the tegument and its vital functions.\udConclusions: This study provided the evidence for the potential antischistosomal effect of\udcompound BTP-Iso and its possible uses as an alternative chemotherapeutic agent for treatment\udof schistosomiasis