Open AccessRecord of porcine brucellosis in India by indigenously developed indirect ELISA
Author(s) -
Rajeswari Shome,
Triveni Kalleshamurthy,
Padmashree B Shankaranarayana,
Swati Sahay,
N. Kameswara Rao,
Bibek Ranjan Shome,
Jyoti Misri,
Hazir Rahman
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(16)61153-2
Subject(s) - brucellosis , seroprevalence , direct agglutination test , veterinary medicine , medicine , confidence interval , zoonosis , brucella , population , bovine brucellosis , rose bengal , serology , antibody , immunology , brucella abortus , biology , environmental health , genetics
Porcine brucellosis is a contagious and emerging zoonosis but neglected in most of the\udendemic countries including India. The disease in pigs is rarely reported due to non-availability\udof diagnostics or major focus is on bovine brucellosis. Hence, the necessity was felt to develop\udindirect ELISA for the detection of anti-Brucella antibodies and to record spatial seroprevalence\udof porcine brucellosis in the country. The relative diagnostic sensitivity and specificity of the\uddeveloped indirect ELISA were 94.0% and 92.0%, respectively and kappa agreement with rose\udbengal plate test, serum agglutination test and commercial indirect ELISA kit was found to be\ud0.86 (95% confidence interval 0.78–0.93). A total of 2 576 random serum samples sourced\udfrom 10 states were screened by indirect ELISA and true prevalence of 7.2% (95% confidence\udinterval 5.6–8.7) was recorded. The study concluded the prevalence of brucellosis in swine\udpopulation in many states of the country and indirect ELISA as an alternate test to rose bengal\udplate test and serum agglutination tests