Open AccessPropolis-Sahara honeys preparation exhibits antibacterial and anti-biofilm activity against bacterial biofilms formed on urinary catheters
Author(s) -
Saâd Aissat,
Ahmed M. Mustafa,
Noureddine Djebli
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(16)61149-0
Subject(s) - biofilm , microbiology and biotechnology , pseudomonas aeruginosa , staphylococcus aureus , agar , agar diffusion test , chemistry , antibacterial activity , escherichia coli , bacteria , biology , biochemistry , genetics , gene
Objective: To evaluate the antibacterial effect of Sahara honeys (SHs) against bacterial\udbiofilms formed on urinary catheters in combination with propolis-Sahara honeys (P-SHs).\udMethods: Three clinical isolates were subjected to biofilm detection methods. The antibacterial\udand anti-biofilm activity for SHs and P-SHs were determined using agar well diffusion and the\udpercentage of biofilm inhibition (PBI) methods.\udResults: The PBI for Gram-positive bacteria [Staphylococcus aureus (S. aureus)] was in the\udrange of 0%–20%, while PBI for Gram-negative bacteria [Pseudomonas aeruginosa and\udEscherichia coli (E. coli)] were in range of 17%–57% and 16%–65%, respectively. The\udhighest PBI (65%) was produced by SH2 only on E. coli. In agar well diffusion assay, zones\udof inhibition ranged from 11–20 mm (S. aureus), 9–19 mm (Pseudomonas aeruginosa) and\ud11–19 mm (E. coli). The highest inhibition (20 mm) was produced by SH1 only on S. aureus.\udIn addition, the treatment of SHs and P-SHs catheters with a polymicrobial biofilms reduced\udbiofilm formation after 48 h exposure period.\udConclussions: SHs and P-SHs applied as a natural agent can be used as a prophylactic agent to\udprevent the formation of in vitro biofilm