Open AccessDiagnosis of acute toxoplasmosis in pregnant women referred to therapeutic centers of Alborz Province (Iran) using immunoglobulin G avidity ELISA technique
Author(s) -
Lame Akhlaghi,
Fatemeh Tabatabaie,
Ramtin Hadighi,
Fatemeh Maleki,
Fateme Hajialiani,
Mohammad Saaid Dayer,
Amir Ghasemi,
Masoud Roudbari
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(16)61147-7
Subject(s) - avidity , toxoplasmosis , titer , toxoplasma gondii , medicine , antibody , immunology , immunoglobulin g , immunoglobulin m
Objective: To evaluate immunoglobulin G (IgG) avidity as a useful and reliable technique\udin diagnosing toxoplasmosis in pregnant women referring to therapeutic centers of Alborz\udProvince (Iran) in 2014, against two other tests, IgG and immunoglobulin G (IgM) antiToxoplasma.\udMethods: Serum samples (468 in total) were obtained from different therapeutic centers in\udKaraj City. ELISA method was used to test the anti-Toxoplasma avidity of IgG, IgM and IgG.\udThe data were analyzed by descriptive statistical methods and Chi-square test (P < 0.05) using\udSPSS 17.0.\udResults: Anti-Toxoplasma tests of IgM and IgG were positive in 9 and 86 samples respectively.\udAlso, a borderline IgM was detected in 2 suspected samples. In addition, among all positive\udand suspected samples, 79 cases indicated high titers of IgG avidity, 7 cases were of low\udtiters and 1 case was of a borderline titer. The prevalence of anti-Toxoplasma antibodies was\ud20%. The sera which showed high avidity index was obtained from patients at chronic phase\udof infection (92%) while those which showed low avidity levels were from patients at acute\udtoxoplasmosis (77.7%).\udConclusions: This study clearly showed that acute and chronic phases of toxoplasmosis could\udbe differentiated with the aid of IgG avidity test. This test may also assist in recognizing old\udand newly acquired infections