Open AccessAntibacterial effect of silver nanoparticles and capsaicin against MDR-ESBL producing Escherichia coli: An in vitro study
Author(s) -
Debasish Kar,
Samiran Bandyopadhyay,
Umesh Dimri,
Deba Brata Mondal,
Pramod Kumar Nanda,
Arun K. Das,
Subhasish Batabyal,
Premanshu Dandapat,
S. Bandyopadhyay
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(16)61135-0
Subject(s) - capsaicin , minimum inhibitory concentration , escherichia coli , silver nanoparticle , chemistry , microbiology and biotechnology , multiple drug resistance , antimicrobial , antibacterial activity , antibiotics , bacteria , biology , nanoparticle , biochemistry , materials science , nanotechnology , receptor , genetics , gene
Objective: To evaluate the antibacterial property of silver nanoparticles (AgNPs) and capsaicin\udagainst multidrug resistant (MDR) and extended spectrum beta-lactamase (ESBL) producing\udEscherichia coli of bovine and poultry origin.\udMethods: Antibacterial efficacy of AgNPs and capsaicin was measured using broth dilution\udmethod. Five MDR-ESBL producing E. coli isolates of poultry (PEC4, PEC6, PEC15 and PEC16)\udand cattle mastitis origin (MEC2) were taken to evaluate the antibacterial effect of AgNPs and\udcapsaicin.\udResults: At 50 mmol/L AgNPs, the viability of MDR of bacterial pathogens was reduced to\udalmost 80%–90% and at 1000 mmol/L, the viability went down to 0%–3%. The minimum\udinhibitory concentration (MIC50) of AgNPs against these MDR-ESBL producing isolates was\udfound to vary between 172–218 mmol/L whereas the MIC80 varied between 450–640 mmol/L.\udCapsaicin showed more prominent bactericidal effect and only at 2.5 mmol/L concentration,\udthe viability was shown to be reduced by 20%–35% whereas at 7.5 mmol/L concentration,\udthere was approximately 60% reduction in viability. Further at 25 mmol/L concentration,\udthe viability was reduced to 0%–8%. The MIC50 and MIC80 of capsaicin against these MDRESBL producing isolates were found to vary between 4.6–7.5 mmol/L and 10.9–16.9 mmol/L,\udrespectively.\udConclusions: The results point out that capsaicin and AgNPs could be of use in treating ESBL\udinfection