Open AccessIn vitro antibacterial and antifungal activities of twelve sponges collected from the Anambas Islands, Indonesia
Author(s) -
Masteria Yunovilsa Putra,
Tri Aryono Hadi,
Tutik Murniasih
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(16)61119-2
Subject(s) - sponge , antimicrobial , aspergillus niger , candida albicans , broth microdilution , microbiology and biotechnology , staphylococcus aureus , bacteria , biology , antibacterial activity , bacillus subtilis , gram positive bacteria , minimum inhibitory concentration , food science , botany , genetics
Objective: To evaluate antimicrobial activities in methanolic extracts of twelve sponges\udcollected from the Anambas Islands, Indonesia.\udMethods: The antibacterial activity of methanolic extracts was tested against two Grampositive bacteria, viz. Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 25923),\udand two Gram-negative bacteria, viz. Eschericia coli (ATCC 25922) and Vibrio anguillarum\ud(ATCC 19264) using the disk diffusion assay. The antifungal activity was similarly tested\udagainst Candida albicans (ATCC 10231) and Aspergillus niger (ATCC 16404). The minimum\udinhibitory concentrations of promising sponges extracts were determined by the microdilution\udtechnique.\udResults: All the sponge species in this study showed antimicrobial activities against at least\udone of the test strains. Antibacterial activities were observed in 66.7% of the sponges extracts,\udwhile 30.0% of the extracts exhibited antifungal activities. Among them, the extracts of the\udsponges Stylissa massa and Axinyssa sp. were the most active against four tested bacteria and\udthe yeast Candida albicans. The sponge Theonella swinhoei and two species of Xestospongia\udalso displayed significant activities against two fungal pathogens Candida albicans and\udAspergillus niger.\udConclusions: Antimicrobial activities were demonstrated in extracts from various marine\udsponges collected from the Anambas Islands, Indonesia. The most promising sponges among\udthem were Stylissa massa and Axinyssa sp. This is the first report of antimicrobial activity in\udextracts of marine sponges from the Indonesian Anambas Islands