Open AccessMelissa officinalis efficacy against human influenza virus (New H1N1) in comparison with oseltamivir
Author(s) -
Parvane Jalali,
Afagh Moattari,
Ali Mohammadi,
Nima Ghazanfari,
Gholamhosein Pourghanbari
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(16)61115-5
Subject(s) - oseltamivir , titer , virus , hemagglutination assay , influenza a virus , virology , microbiology and biotechnology , inoculation , hemagglutination , cell culture , melissa officinalis , cytotoxicity , mtt assay , chemistry , in vitro , biology , traditional medicine , medicine , immunology , biochemistry , covid-19 , genetics , disease , pathology , infectious disease (medical specialty)
Objective: To evaluate the antiviral activity of Melissa officinalis (MO) extract against the\udinfluenza virus H1N1 in vitro.\udMethods: The cytotoxicity of MO extract was identified on Madin-Darby canine kidney\ud(MDCK) cell culture by MTT assay. The virus was inoculated to the cells (multiplicity of\udinfection = 0.1) in two protocols. In protocol 1, the MO extracts at concentrations of 0.005,\ud0.050, 0.010, 0.100 and 0.500 mg/mL were incubated with the virus for one hour preinoculation. In protocol 2, the mentioned concentrations of MO extracts were added to the\udcells one-hour post infection. Furthermore, the antiviral effect of oseltamivir with different\udconcentrations was tested as the positive controls. The 50% tissue culture infective dose,\udneutralizing index and hemagglutination titer were determined.\udResults: The medicine oseltamivir and MO extracts were not toxic for MDCK at concentrations\udless than 1 mg/mL. All utilized concentrations of MO extracts were vigorously efficient to\uddecrease the viral yield in both experiments. The 50% tissue culture infective dose of the\udgroups containing up to 0.100 mg/mL of MO extracts in the first experiment in compare with\ud0.050 mg/mL in the second experiment reduced to 0. Although hemagglutination tests showed\udlittle titers, the viral quantity significantly decreased in both experiments. By the way, the\udmedicine oseltamivir could completely suppress viral replication in MDCK.\udConclusions: The present study suggests that MO extracts have a potent anti-influenza effect in\udcell culture