Open AccessPhenolic profiling of Rorippa palustris (L.) Besser (Brassicaceae) by LC-ESI-MS: Chemosystematic significance and cytotoxic activity
Author(s) -
Mona M. Marzouk,
Sameh R. Hussein,
Ahmed Elkhateeb,
Mai M. Farid,
Lamyaa F. Ibrahim,
ElSayed S. AbdelHameed
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(16)61100-3
Subject(s) - kaempferol , quercetin , botany , biology , electrospray ionization , chemistry , brassicaceae , chromatography , traditional medicine , mass spectrometry , biochemistry , antioxidant , medicine
Objective: To investigate the phenolic profile of Rorippa palustris (R. palustris) compared\udto Egyptian Rorippa species to evaluate their chemosystematic significance as well as screen\udcytotoxicity of R. palustris extract.\udMethods: The chemical components of defatted hydroalcoholic R. palustris extract were\udanalyzed using liquid chromatography-electrospray ionization mass spectrometry technique.\udThe cytotoxic activity was evaluated by using MTT assay against four carcinoma cell lines.\udResults: Sixteen compounds corresponding to flavonoids (kaempferol and quercetin\udderivatives), phenolic acids (gallic, coumaric and ferulic acids derivatives) and an anthocyanin\ud(delphinine derivative) were identified or tentatively characterized, of which ten compounds\udwere detected for the first time from R. palustris. Moreover, R. palustris extract showed a\udmoderate activity against MCF7 and A549 cell lines at 100 mg/mL with cell viability of 47.3%\udand 65.4%, respectively.\udConclusions: The phenolic profile and the morphological characters of R. paluatris seem to be\udin relation with those of Rorippa indica, which can be considered as an indicative parameter\udfor its medicinal importance