Open AccessDevelopment and validation of a meat juice ELISA for the diagnosis of Fasciola hepatica in cattle in Cuba
Author(s) -
Amílcar Arenal,
Vladimir López,
Arnielis Díaz,
Stijn Casaert,
A. Arteaga,
Yipsy Santiago García,
Efren Menendez,
Yuliet Sánchez,
Jozef Vercruysse,
Johannes Charlier
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(16)61097-6
Subject(s) - fasciolosis , fasciola hepatica , repeatability , hepatica , antibody , veterinary medicine , biology , food science , chromatography , chemistry , medicine , immunology , helminths
Objective: To establish and validate a home-made ELISA for determination of antibodies\udagainst excretory-secretory proteins of Fasciola hepatica in bovine meat juice samples.\udMethods: The validity criteria of the assay were defined based on standards of ISO. The\udfollowing parameters were evaluated: excretion/secretion antigen concentrations for coating,\udanti-bovine immunoglobulin G dilution, linearity, accuracy and precision.\udResults: The assay was validated on 126 meat juice samples with known infection status.\udUsing the receiver operating characteristic (n = 126) the optimal cut-off for the ELISA assay\udwas 0.78, above this value the probability for an animal to have fasciolosis was 11 times. And\udthe specificity and sensitivity were 100% and 90.91% respectively. The repeatability of the\udintra- and inter-assay tests had coefficients of variation lower than 10% and 20% respectively.\udConclusions: The ELISA is a suitable test for further use in studies towards the epidemiology\udof Fasciola hepatica in Cuba