Open AccessEvaluation of the frequency of precore/core mutation in patients with chronic hepatitis B, Kerman, Southeast of Iran
Author(s) -
Fariba Soleimani,
Seyed Ali Mohammad Arabzadeh,
Hamid Reza Mollaei,
Zahra Iranmanesh,
Najmeh Nikpour,
Minoo Motahar
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(16)61093-9
Subject(s) - hepatitis b virus , virology , medicine , mutation , serology , hepatitis b , chronic hepatitis , antigen , point mutation , hepatitis , polymerase chain reaction , virus , immunology , antibody , biology , gene , genetics
Objective: To evaluate the frequency of precore/core mutation in patients with chronic hepatitis\udB using PCR-restricted fragment length polymorphisms method.\udMethods: Sera were obtained from 69 patients with chronic hepatitis B including 30 women\ud(43.5%) and 39 men (56.5%). All patients were tested for the serum alanine aminotransferase,\udaspartate aminotransferase, the presence of hepatitis Be antigen and hepatitis B surface antigen\udby electrochemiluminescence and hepatitis B virus DNA load. Precore/core mutation was\udexamined for the presence of a characteristic point mutation at nucleotides A1896G in precore\udand A1762T and G1764A in core region using nested PCR and restricted fragment length\udpolymorphisms methods.\udResults: From total 69 cases with chronic hepatitis B infection, 12 (17.3%) patients had\udprecore mutation and 10 (14.4%) patients had core mutation. From 69 patients, 53 (76.8%)\udwere negative for hepatitis Be antigen and 61 (88.4%) were positive for hepatitis B surface\udantigen by electrochemiluminescence method. All samples were positive for hepatitis B virus\udDNA by RT-PCR.\udConclusions: This study suggests that extra molecular methods should be apply for diagnosis\udand monitoring of mutation in chronic hepatitis B patients synchronic or serological method