Open AccessBiological application of green silver nanoparticle synthesized from leaf extract of Rauvolfia serpentina Benth
Author(s) -
Sudipta Panja,
Indranil Chaudhuri,
Kalyani Khanra,
Nandan Bhattacharyya
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(16)61085-x
Subject(s) - silver nanoparticle , fourier transform infrared spectroscopy , hela , nuclear chemistry , spectroscopy , nanoparticle , transmission electron microscopy , chemistry , surface plasmon resonance , infrared spectroscopy , energy dispersive x ray spectroscopy , selected area diffraction , cytotoxicity , materials science , scanning electron microscope , nanotechnology , organic chemistry , chemical engineering , biochemistry , physics , quantum mechanics , engineering , in vitro , composite material , cell
Objective: To synthesize silver nanoparticles (AgNPs) from the leaf extract of Rauvolfia\udserpentina Benth and examination of their various biological activities.\udMethods: An ecofriendly, easy, one step, non-toxic and inexpensive approach is used, where\udaqueous plant extract acts as a reducing as well as stabilizing agent of AgNPs. The nanoparticles\udwere characterized by UV-vis spectroscopy, Fourier transform infrared spectroscopy, transmission\udelectron microscopy, X-ray diffraction, and energy-dispersive X-ray spectroscopy analysis.\udResults: Surface plasmon resonance of the nanoparticles was observed at 427 nm in UV-vis\udspectroscopy. Fourier transform infrared spectroscopy result confirms that the plant extract acts\udas the reducing as well as the capping agent of the AgNPs. Transmission electron microscopy\udindicated that the synthesized nanoparticles are spherical in shape and approximately 7–10\udnm in size, whereas the crystalline nature with face-centered cubic structure of the AgNPs\udwas detected by X-ray diffraction analysis. Presence of silver in the AgNPs is 31.43% by\udweight, as confirmed by energy-dispersive X-ray spectroscopy. The synthesized AgNPs have\udantimicrobial activities against human pathogenic microorganisms. It also shows larvicidal\udactivity and cytotoxicity against HeLa, MCF-7 cell lines.\udConclusions: Synthesized spherical shaped AgNPs from the leaf extract of Rauvolfia\udserpentina Benth have antimicrobial and larvicidal activities as well as cytotoxicity against\udHeLa and MCF-7 cell lines