Open AccessEvaluation of HBV resistance to tenofovir in patients with chronic hepatitis B using ZNA probe assay in Kerman, southeast of Iran
Author(s) -
Minoo Motahar,
Seyed Alimohammad Arabzadeh,
Hamid Reza Mollaei,
Zahra Iranmanesh,
Najmeh Nikpour,
Fariba Soleimani
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(16)61079-4
Subject(s) - hbeag , hbsag , hepatitis b virus , virology , hepatitis b virus dna polymerase , hepatitis b , mutation , medicine , polymerase chain reaction , polymerase , serology , drug resistance , microbiology and biotechnology , virus , gene , biology , immunology , antibody , genetics
Objective: To evaluate the mutation rate of polymerase gene and it’s correlation with tenofovir\udresistance in patients with chronic hepatitis B.\udMethods: A total of 64 serum samples (36 men and 28 women) were collected from patients\udwith chronic hepatitis B. All of these samples were tested for hepatitis B virus (HBV) DNA\udlevel, alanin amino transferase/aspartic amino transferase enzymes and serological markers\udsuch as hepatitis B surface antigen (HBsAg)/hepatitis Be antigen (HBeAg) (Electro-chemiluminescence).\udResults: In this study, out of those 64 patients, 13 cases had mutations in the polymerase\udregion (A194T). All mutant cases were HBsAg positive and 5 (38.5%) of them were males and\ud8 (61.5%) were females, while 6 (46.2%) of the mutants were HBeAg positive and 7 (53.8%)\udwere HBeAg negative and most of the mutants have more than 109 HBV DNA level. Statistical\udanalyses on the 64 samples showed that there was no significant relation between age and\udHBsAg level but there was a significant relationship between HBV DNA load (P = 0.001) and\udA194T mutation.\udConclusions: HBeAg had a correlation with A194T mutation (P = 0.02) and tenofovir\udresistance was seen in 13 patients. Real-time PCR with zip nucleic acid probes is a rapid\udmethod to detect mutations in the polymerase region of HBV with high sensitivity and specifity.\udThis method could be used for mutation detection in nt-194 position of polymerase gene for\udtenofovir resistance and other mutations in drug resistance researches