Open AccessRepetitive sequence-based PCR fingerprinting and the relationship of antimicrobial-resistance characteristics and corresponding genes among Salmonella strains from pig production
Author(s) -
Teerarat Prasertsee,
Nattakarn Khantaprab,
Panuwat Yamsakul,
Pannita Santiyat,
Nipa Chokesajjawatee,
Prapas Patchanee
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)61054-4
Subject(s) - biology , salmonella , microbiology and biotechnology , tetracycline , nalidixic acid , streptomycin , antibiotic resistance , antimicrobial , sulfamethoxazole , ampicillin , multiple drug resistance , drug resistance , antibiotics , genetics , bacteria
Objective: To investigate the relationship between antimicrobial resistance characteristics\udand corresponding genes, and to diversify repetitive element sequence-based PCR (rep-PCR)\udfingerprinting of three Salmonella serotypes: Rissen, Panama and Stanley, which were isolated\udfrom pig farms and slaughterhouses in Chiang Mai and Lumphun Provinces, Thailand.\udMethods: A total of 90 Salmonella strains were identified using the Kauffman-White scheme.\udThe Kirby-Bauer disk diffusion method was used to investigate resistance phenotypes of 10\udantimicrobial agents. Conventional PCR was used to detect 10 antimicrobial resistance genes,\udadditionally, rep-PCR typing method was applied to identify clonality among Salmonella\udisolates.\udResults: The antimicrobial susceptibility testing found resistance to ampicillin (80.0%),\udstreptomycin (65.6%), tetracycline (61.1%), sulfamethoxazole (53.3%), chloramphenicol\ud(28.9%), nalidixic acid (6.7%) and cefotaxime (2.2%). All strains were sensitive to amoxicillinclavulanic acid, ciprofloxacin and norfloxacin. The most common antimicrobial resistance\udpatterns among the isolates were ampicillin, chloramphenicol, streptomycin, tetracycline, and\udsulfamethoxazole. The type and frequency of antimicrobial genes detected included blaTEM\ud(100.0%), aadA2 (52.2%), cmlA (45.6%), strA (38.9%), tetA(B) (16.7%), sul1 (15.6%),\udbla\udOXA-2 (14.4%), blaPSE1 (6.7%), aphA1-lab (2.2%) and blaCMY-2 (1.1%).\udConclusions: Statistical analysis revealed no association between antimicrobial resistance\udgenes and resistance profiles with the exception of cmlA and chloramphenicol, sul1 and\udsulfamethoxazole, aadA2 and streptomycin, and strA and streptomycin (P < 0.05). Finally,\udthe identical groups of clonal strains were detected by rep-PCR indicating similarity among\udSalmonella genotypes from farms and from slaughterhouses