Open AccessInvestigation on prevalence of Escherichia coli strains carrying virulence genes ipaH, estA, eaeA and bfpA isolated from different water sources
Author(s) -
Reza Ranjbar,
Shera Hosseini,
Taghi Zahraei-Salehi,
Roholla Kheiri,
Faham Khamesipour
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)61031-3
Subject(s) - virulence , escherichia coli , ethidium bromide , microbiology and biotechnology , plasmid , gene , biology , amplicon , agarose gel electrophoresis , polymerase chain reaction , dna , genetics
Objective: To investigate prevalence of Escherichia coli (E. coli) strains carrying virulence\udgenes ipaH, estA, eaeA and bfpA, isolated from different water sources in Alborz Province.\udMethods: This study was carried out in 2014. The research included all E. coli strains isolated\udfrom different surface water sources in Alborz Province of Iran. E. coli isolates were detected\udand identified by standard microbiological and biochemical tests. The strains were evaluated\udfor the presence of virulence genes ipaH, estA, eaeA and bfpA by PCR using specific primers.\udThe PCR amplicons were visualized via electrophoresis and stained with ethidium bromide.\udResults: One hundred E. coli strains were isolated and included in the study. The PCR results\udshowed that 97% of the strains harbored ipaH gene. Moreover, estA, eaeA and bfpA genes were\udfound in 37%, 31% and 3% of the isolates.\udConclusions: Our finding showed that the prevalence rates of virulence genes ipaH and estA\udwere very high among E. coli strains isolated from different surface water sources in Alborz\udProvince. Considering their plasmid-borne nature, the risk of transmission of these genes\udbetween other bacterial species could pose a high threat to public health