Open AccessEvidence of stable insulin and its increased efficacy during oral administration with Desmodium gangeticum extract in rats
Author(s) -
Siddhar Selvam Gandhi,
Vidyasagar Saranya,
Karthikeyan Krishnasamy
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)61016-7
Subject(s) - insulin , diabetes mellitus , medicine , oral administration , homeostasis , endocrinology , streptozotocin , pharmacology , chemistry
Objective: To find the efficacy of administering insulin orally with Desmodium gangeticum\ud(DG) extract in diabetes induced rats.\udMethods: Diabetes was induced in Wistar male rats by streptozotocin and subsequently treated\udwith the mixture of DG/insulin/DG + insulin and compared with diabetic rats. Standard glucose\udestimation assay described elsewhere was used to determine the diabetic levels. The effects of\udhomeostatic factors (the effect of temperature, gastric juices, pH, trypsin, and mercaptoethanol)\udon insulin and insulin-DG were analyzed.\udResults: The test results proved that the reported combination of insulin-DG was effective in\udmaintaining glucose homeostasis. More interestingly, insulin-DG was stable against all the\uddegrading parameters while insulin without DG suffered degradation. A molecular interaction\udwas suggested between DG and insulin through nuclear magnetic resonance and Fourier\udtransform infrared spectroscopy analysis.\udConclusions: Insulin-DG mix is a potent anti-diabetic agent and importantly, DG extract is\udprotective to insulin when used in prescribed combination and delivered orally. This could be a\udpossible solution for painful subcutaneous injections