Open AccessToxoplasma gondii in women with recent abortion from Southern Mexico
Author(s) -
Ivonne Beatriz Hernández-Cortazar,
Karla Y. Acosta-Viana,
Eugenia Guzmán-Marı́n,
José Candelario Segura-Correa,
Antonio OrtegaPacheco,
Jorge Ramiro Carrillo-Martínez,
Marı́a del Refugio González-Losa,
Laura Conde-Ferráez,
Matilde JiménezCoello
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)61012-x
Subject(s) - seroprevalence , avidity , toxoplasma gondii , serology , odds ratio , abortion , confidence interval , immunology , toxoplasmosis , parasitemia , biology , medicine , virology , antibody , pregnancy , malaria , plasmodium falciparum , genetics
Objective: To estimate the presence, parasite burden, percentage of avidity and risk factors to\udseropositivity for Toxoplasma gondii (T. gondii) in women with recent abortion attended at the\udtwo main hospitals from Merida, Yucatan, Mexico.\udMethods: Seroprevalence and IgG avidity were estimated from 161 blood samples from\udaborting women and risk factors associated with serological status were determined. Only\ud130 from 161 samples were analyzed by quantitative PCR (qPCR) and a nested-PCR (nPCR) to\uddetect T. gondii-DNA.\udResults: Seroprevalence of Toxoplasma IgM and IgG was 3.72% (6/161) and 59.00% (95/161),\udrespectively; 98.94% (94/95) of the women showed high-avidity index and only 1.05%\ud(1/95) demonstrated low-avidity. For qPCR, 3.84% (5/130) of the samples were detected with\udT. gondii-DNA and all positive samples showed a low parasite load (< 1 parasite/mL). For\udnPCR, 6.92% (9/130) of positivity was found. Logistic regression analysis showed a positive\udassociation between IgG-seropositivity and potable-drinking-water intake in households (P <\ud0.020, odds ratio = 11.62, 95% confidence interval = 0.011–0.683).\udConclusions: In this study, the detection of T. gondii-DNA in IgG-positive women suggests in\udsome cases active parasitemia, even when they demonstrated high-avidity index. Reinfection\udcases could be presented due to the high circulation of the parasite in the study region. T.\udgondii could play an important role as cause of abortion and municipal potable-water could be\udan important risk factor of infection