Exploiting the role of various types of ion-channels against chemically induced inflammatory bowel disease in male Wistar rats

Objective: To evaluate the role of nifedipine (L-type of calcium channel blocker),\udglibenclamide [cystic fibrosis transmembrane conductance regulator (CFTR) channel blocker],\udniflumic acid (chloride channels blocker) and 2-aminoethyldiphenyl borate (calcium releaseactivated channels channel blocker) against chemically induced inflammatory bowel disease in\udmale Wistar rats.\udMethods: Inflammatory bowel disease was induced by the administration of 2,4-dinitrobenzene\udsulfonic acid intracolonically. Animals were divided into 7 groups by keeping 6 animals in\udeach group. Group one and two were treated as normal control and disease control, respectively\udand seven pretreated with sulfasalazine and remaining groups were treated with test drugs.\udAnimals were pretreated for 5 days before administration of 2,4-dinitrobenzene sulfonic acid.\udVarious parameters were recorded during and after treatment period like disease activity index\ud(body weight, food intake, diarrhea score, occult blood score), biomarkers study in peritoneal\udmacrophages and histopathology of colon.\udResults: Glibenclamide had offered significant protective effect on disease activity index,\udbiochemical parameters confirmed by histopathological examination. 2-Aminoethyldiphenyl\udborate and niflumic acid had also shown insignificant protection in few parameters.\udConclusions: Protection observed by pretreatment of glibenclamide is may be of its\udblocking effect of CFTR channels of the mast cells, which regulate chloride influx and efflux\udphenomenon