Open AccessPhytochemical analysis and antimicrobial activity of some medicinal plants against selected common human pathogenic microorganisms
Author(s) -
Divya Gupta,
Jyotirmay Dubey,
Mukesh Kumar
Publication year - 2016
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)60978-1
Subject(s) - phytochemical , antimicrobial , traditional medicine , agar diffusion test , staphylococcus aureus , antibacterial activity , chemistry , terpenoid , petroleum ether , candida albicans , glycoside , saponin , microbiology and biotechnology , biology , bacteria , botany , biochemistry , medicine , genetics , alternative medicine , chromatography , pathology , extraction (chemistry)
Objective: To study the antimicrobial activity and phytochemicals of extracts from 5 different\udmedicinal plants, as well as to evaluate the synergistic activity of potent plant extracts with\udsuitable antibiotic discs and antibiotics susceptibility of tested microorganisms.\udMethods: The antimicrobial activities of different extracts were evaluated by using agar well\uddiffusion method and antibiotics susceptibility of five selected microorganisms was tested\udby using disc diffusion method. For determination of synergistic activities of the potent plant\udextracts along with antibiotic discs, agar well diffusion and disc diffusion methods were\udcombinedly used.\udResults: In the present investigation, the maximum in vitro inhibition of tested microorganisms,\udEscherichia coli, Pseudomonas aeruginosa, Candida albicans and Staphylococcus aureus\udwas recorded in chloroform and methanol extracts of Terminalia arjuna, ethanol extract\udof Camellia sinensis, and petroleum ether extract of Polyalthia longifolia which offered\udinhibition zone ranged from 11 to 18 mm. The maximum antibacterial efficacy was exhibited\udby levofloxacin with an inhibition zone of 35 mm against Escherichia coli. The potent plant\udextracts showed positive synergistic effects against Staphylococcus aureus with lincomycin.\udThe phytochemical analysis of the potent plant extracts revealed the presence of saponin,\udtannin, protein, carbohydrate, flavonoid, terpenoids and glycosides.\udConclusions: According to the present study, Camellia sinensis, Terminalia arjuna and\udPolyalthia longifolia can be used as a potent source of natural antimicrobial agents by replacing\udcommercially available synthetic drug that may have a large number of side effects