Open AccessPhysical, chemical and biological studies of gelatin/chitosan based transdermal films with embedded silver nanoparticles
Author(s) -
Sneha Paul,
Aiswarya Jayan,
Changam Sheela Sasikumar
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)60968-9
Subject(s) - transdermal , chitosan , silver nanoparticle , permeation , nuclear chemistry , gelatin , chemistry , nanoparticle , materials science , chemical engineering , nanotechnology , membrane , organic chemistry , medicine , engineering , pharmacology , biochemistry
Objective: To study the physical, chemical and biological properties of composite chitosangelatin transdermal film along with silver nanoparticles as binding agent and determine the\udcompatibility of the prepared amalgamation towards wound management.\udMethods: Transdermal film preparations were done by solvent casting method containing\uddifferent concentrations of biological synthesized silver nanoparticles. The films were\udcharacterized by using scanning electron microscope for their morphology and the\uddetermination of silver metal was done by using inductively coupled plasma atomic emission\udspectroscopy. Then a quantity of silver nanoparticles was further proceeded by physiochemical\udparameters (weight, thickness, temperature, solubility, absorption, tensile strength, in vitro drug\udrelease and skin permeation) and biological parameters studies (anti-microbial, cytotoxicity\udand reactive oxygen species).\udResults: The film prepared by utilizing 2 g of gelatin and 0.5 g of chitosan exhibited better\udresults. The physiochemical parameters studies revealed higher concentration of silver\udnanoparticles would give better results. In vitro drug release studies through dialysis and\udskin permeation showed the release of drug versus time (h). These films had shown excellent\udinhibition against Streptococcus and Escherichia coli species. Cytotoxicity study by MTT\udindicated the mild toxicity existed as the concentration of silver nanoparticles increased.\udReactive oxygen species generation studies of transdermal film by using 2'7'-dichlorofluorescein\uddiacetate assay demonstrated that the fluorescent cells were found in the higher concentration,\udwhich indicated cell damage (reactive oxygen species generated).\udConclusions: Based on these observations, in vitro performances against various characteristics\udof transdermal film, would be utilized as a distinct dressing material and patches accessible in\udmarket