Open AccessIn vitro evaluation of antioxidant and antidiabetic activities of Syzygium densiflorum fruits
Author(s) -
Krishnasamy Gopinath,
Karthikeyan Muthusamy
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)60956-2
Subject(s) - chemistry , phytochemical , ethyl acetate , antioxidant , hydroxyl radical , ethanol , tbars , food science , dpph , phenols , lipid peroxidation , terpenoid , traditional medicine , chromatography , organic chemistry , biochemistry , medicine
Objective: To provide experimental support for the traditional knowledge of Syzygium\uddensiflorum (S. densiflorum) fruits.\udMethods: Powered S. densiflorum dried fruits were subjected to successive extraction\udwith n-hexane, ethyl acetate, and ethanol using a Soxhlet extractor. Further, preliminary\udphytochemical screening was carried out with a series of tests. In vitro free radical scavenging\udwas evaluated using total antioxidant estimation, 2,2-diphenyl-1-picrylhydrazyl radical,\udsuperoxide radical scavenging, and hydroxyl radical scavenging assays. Antidiabetic activity\udwas estimated using α-amylase inhibition assay.\udResults: Preliminary phytochemical estimation confirmed the presence of alkaloids,\udflavonoids, sterols, terpenoids, anthocyanin, phenols, carbohydrates, fixed oils, and fats in fruits\udof S. densiflorum. Ethyl acetate and n-hexane extracts showed less free radical scavenging\udand α-amylase inhibition activity than ethanol extract. IC50 values of ethanol extracts for 2,2-\uddiphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, lipid peroxidation,\udand α-amylase inhibition assays were found to be 0.01, 0.16, 0.66, 0.46, and 0.46 mg/mL\udrespectively.\udConclusions: In vitro evaluations confirmed the antioxidant and antidiabetic potential of\udS. densiflorum fruits. Ethanol extract of S. densiflorum fruits showed higher activity with\udstatistical significance vs. ethyl acetate and n-hexane extracts