Open AccessEvaluation and comparison of polyphenols and bioactivities of wild edible fruits of North-West Himalaya, India
Author(s) -
Har Lal Singh,
Madhuri Kaushish Lily,
Koushalya Dangwal
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)60951-3
Subject(s) - traditional medicine , chemistry , elastase , gallic acid , botany , dpph , polyphenol , tyrosinase , flavonoid , antioxidant , food science , biology , biochemistry , enzyme , medicine
Objective: To evaluate and compare the polyphenol contents, antioxidant, anti-elastase,\udanti-collagenase, anti-tyrosinase and anti-inflammatory activities of 13 wild edible fruits\ud[Pyracantha crenulata, Berberis asiatica (B. asiatica), Ficus subincisa (F. subincisa), Morus\udserrata, Ziziphus nummularia, Leea asiatica (L. asiatica), Dendrobenthamia capitata, Ziziphus\udmauritiana, Prunus cerasoides, Ampelocissus latifolia (A. latifolia), Vitis jacquemontii, Morus\udalba and Grewia optiva] of North-West Himalayan Region of India.\udMethods: Fruits extracts were prepared with 80% aqueous acetone and evaluated for total\udphenolic contents (TPC) and total flavonoid contents (TFC). Free radical scavenging activities\ud[against 1,1-diphenyl-2-picryl-hydrazyl, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),\udlinoleate hydroperoxyl and superoxide radicals], ferric reducing ability, ferrous metal chelating\udcapacity, anti-elastase, anti-collagenase, anti-tyrosinase and anti-inflammatory activities were\uddetermined by using various in vitro assays.\udResults: TPC varied from 58.83 to 4 496.39 mg gallic acid equivalents/100 g fruit weight (FW),\udbeing highest in A. latifolia and lowest in F. subincisa. TFC ranged from 108.00 to 1 963.75 mg\udcatechin equivalents/100 g FW, standing highest in L. asiatica and lowest in Prunus cerasoides.\udA. latifolia and L. asiatica possessed the highest antioxidant activities while B. asiatica and L.\udasiatica owned uppermost anti-elastase and anti-collagenase activities, respectively. B. asiatica\udrevealed the highest anti-tyrosinase activity and F. subincisa demonstrated the highest antiinflammatory activity. The present study revealed differential contribution of TPC and TFC\udin various antioxidant activities. However, no obvious relationship was visible between antielastase/anti-collagenase/anti-tyrosinase/anti-inflammatory activities and TPC/TFC, suggesting\udthe role of individual or combination of specific phenolics and flavonoids.\udConclusions: The abilities of Himalayan wild edible fruits to scavenge a variety of free\udradicals, inhibit enzymes causing skin-aging and skin-darkening are highly appreciable,\udsuggesting their possible utilization for the development of effective formulations for general\udhealth maintenance and anti-aging, skin-whitening cosmetics