Open AccessFaunal identification and frequency distribution of wild sand flies infected with Leishmania tropica
Author(s) -
Mohammad Djaefar MoemenbellahFard,
Mohammad Ahmadyousefi-Sarhadi,
Kourosh Azizi,
Mohammad Reza Fakoorziba,
Mohsen Kalantari,
Masoumeh Amin
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)60932-x
Subject(s) - phlebotomus , leishmania tropica , biology , vector (molecular biology) , leishmania , veterinary medicine , genus , cutaneous leishmaniasis , leishmania major , leishmaniasis , psychodidae , zoology , parasite hosting , immunology , medicine , biochemistry , world wide web , computer science , gene , recombinant dna
Objective: To determine the sand fly composition, its frequency distribution and natural\udinfection with Leishmania parasites to incriminate the likely vectors of cutaneous leishmaniosis\udin Jiroft, Southeast Iran.\udMethods: Sand flies were caught with sticky paper traps during a 6-month period in 2013.\udThey were morphologically identified to species level using valid taxonomic keys. They were\udthen subjected to nested PCR method and the results were analyzed.\udResults: A total of 3 751 sand flies were identified to belong to 21 species in two genera (8 spp.\udin the Phlebotomus genus, and 13 spp. in the Sergentomyia genus) most of which were males\ud(65.60%) and exophilic (63.80%). The two most frequent species were Phlebotomus papatasi\ud(39.40%) and Phlebotomus sergenti (17.20%). The latter was confirmed by PCR to be naturally\udinfected with Leishmania tropica (3.33%).\udConclusions: It was thus concluded that wild-caught Phlebotomus sergenti naturally infected\udwith Leishmania tropica was mainly incriminated by molecular method to be the principal\udvector of cutaneous leishmaniosis in this endemic focus