Open AccessAcute toxicity of caprine alpha S2-casein protein on the microstructures and mineral profiles of rat ileum
Author(s) -
Vita Agustina,
Bambang Setiawan,
Fatchiyah Fatchiyah
Publication year - 2015
Publication title -
asian pacific journal of tropical disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 33
ISSN - 2222-1808
DOI - 10.1016/s2222-1808(15)60920-3
Subject(s) - ileum , casein , chemistry , jejunum , medicine , perforation , zoology , endocrinology , biology , biochemistry , materials science , punching , metallurgy
Objective: To determine the microstructures and mineral profiles of ileum villi in rats treated\udacutely with caprine αS2-casein (CSN1S2) protein isolated from Etawah goat milk.\udMethods: Fifty (male and female) rats were divided into 5 groups, including a group of ratsuntreated as a control (untreated) and rats-treated with caprine CSN1S2 protein at doses of 500,\ud1 000, 2 000, and 4 000 mg/kg body weight by oral single dosage. The microstructures were\udanalyzed by scanning electron microscope. The mineral profiles of ileum were measured by\udscanning electron microscope-energy dispersive X-ray software version 1.7. The degree of\udileum perforation was calculated by BoneJ software.\udResults: The ileum microstructure of the control group showed a smooth and compact surface,\udwhile the treating groups showed less compact surface and minimal perforation. The levels of\udsodium, sulphur and phosphorus were statistically significant higher in treating females at all\uddoses compared to the control (P < 0.05). In contrast, the selenium levels were significantly\udlower in treating females at all doses than that of the control group (P < 0.05).\udConclusions: This research concludes that the caprine CSN1S2 protein is able to stimulate the\udileum toxicity up to 2 000 mg/kg body weight dosage and change sodium, phosphorus, sulphur\udand selenium homeostasis of ileum in female rats